Information

UniProt ID: P04406

All Names: aging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate  dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685;

Applications: WB IHC ICC/IF IP FC 

 Form: Liquid 

 Storage instructions: Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.  

Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. 

 Purity: Affinity-chromatography  

Immunogen: A synthesized peptide 

 Molecular Wt.: 37 kDa 

Application WB: 1:3000~1:10000 ;IHC: 1:100~1:500 ;IF/ICC: 1:100~1:250 ;IP: 1:50 ;FC: 1:50 

 Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in  glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore  antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors,  such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.  

Western blot analysis of (1) Hela, (2) Jurkat whole cell lysates, (3) Mouse kidney lysates,  (4) Mouse spleen lysates, (5) RAW 264.7 whole cell lysates, (6) Rat brain lysates with GAPDH Antibody.  

For Research Use Only. Not For Use In Diagnostic Procedures.