Information 

UniProt ID: P60709

All Names: ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;  

Applications: WB IHC ICC/IF 

Form: Liquid  

Storage instructions: Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. 

 Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. 

 Purity: Affinity-chromatography  

Immunogen: A synthesized peptide  

Molecular Wt.: 42 kDa

Application 

WB 1:3000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200

Background beta-Actin is one of six different actin isoforms that have been identified. The actin molecules  found in cells of various species and tissues tend to be very similar in their immunological and  physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for  Western Blotting. However it should be noted that levels of beta-Actin may not be stable in  certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it  should not be used as loading control for these tissues. 

Western blot analysis of beta Actin expression in  (1) HeLa cell lysate; (2) Human fetal kidney lysate; (3) NIH/3T3 cell lysate; (4) PC-12 cell lysate.

For Research Use Only. Not For Use In Diagnostic Procedures.